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Image Search Results
Journal: Human Molecular Genetics
Article Title: A neuronal enhancer network upstream of MEF2C is compromised in patients with Rett-like characteristics
doi: 10.1093/hmg/ddy393
Figure Lengend Snippet: MEF2C enhancers display distinct activity patterns. (A) In vitro enhancer activity of candidate elements. Log2 luciferase activity relative to a negative control (NC) vector, in HEK293 (left) and SH-SY5Y (right), for 16 putative enhancer elements (linear mixed effects model, log2 enhancer effect fold change (FC) ± standard error). Green means significant positive effect ( P < 0.01); blue, significant negative effect ( P < 0.01); grey, no significant (NS) effect. (B) Enhancer activity in HEK 293 versus SH-SY5Y cells . Log2 of the relative luciferase activity in HEK293 versus SH-SY5Y cells. (C) Tissue-specific enhancers in zebrafish embryos at 24 and 48 hpf. Eight enhancers induce neuro-specific GFP expression: e1 and e2 in the forebrain and specific peripheral neurons; e3 in the middle of the notochord at 24 hpf; e4 in the forebrain, specific neurons above the eye, somitic muscles and heart; e7 in the forebrain and notochord at 24 hpf; e9 in neurons with vertical projections in the midbrain/hindbrain and spinal cord at 24 hpf e12 drove general GFP expression in the central nerve system, including the head, tail and trunk at 24 hpf; and e15 in the forebrain and notochord at 48 hpf. The color code reflects the observed activity of the 16 tested candidate enhancer elements: green means active in neuronal tissues; blue, active in other than neuronal tissues; white, not active.
Article Snippet: The constructed
Techniques: Activity Assay, In Vitro, Luciferase, Negative Control, Plasmid Preparation, Expressing, Muscles